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Preparation and physical characterization of a homogeneous population of monomeric nucleosomes from HeLa cells.

We describe a method of isolating a homogeneous population of "trimmed" monomeric nucleosomes from Hela cells. These nucleoprotein particles contain a 140 +/- 5 base pair length of DNA and have a histone/DNA ratio of 1.2. They lack H1 and contain equal amounts of the four smaller histones....

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Detalhes bibliográficos
Main Authors: Whitlock, J P, Simpson, R T
Formato: Artigo
Idioma:Inglês
Publicado em: 1976
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC343081/
https://ncbi.nlm.nih.gov/pubmed/967693
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