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Two restriction endonucleases from Bacillus globiggi.
The sites of action of the restriction enzyme Bgl II on lambda DNA are mapped. This enzyme recognises the sequence 5' ...AGATCT...3' and makes staggered cuts producing sticky ends. In lambda DNA, the second A in this sequence is methylated about 50% of the time by a bacterial methylase abs...
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| Hovedforfatter: | |
|---|---|
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
1976
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| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC343033/ https://ncbi.nlm.nih.gov/pubmed/967673 |
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