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Enzymatic synthesis of duplex circular phiX174 DNA containing phosphoramidate bonds in the (-) strand.

Duplex circular phiX174 DNA (RF I) containing some phosphoramidate links in the backbone chain of the (-) strand was synthesized by reaction of 5'-amino-5'-deoxythymidine 5'-triphosphate, dCTP, dGTP, and 3H-dATP with DNA polymerase I and DNA ligase (T4) on a (+) strand phiX174 amber 3...

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Autors principals: Letsinger, R L, Hapke, B, Petersen, G R, Dumas, L B
Format: Artigo
Idioma:Inglês
Publicat: 1976
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC342964/
https://ncbi.nlm.nih.gov/pubmed/775443
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