In vivo protein crystallization opens new routes in structural biology

Protein crystallization in cells has been observed several times in nature. However, owing to their small size these crystals have not yet been used for X-ray crystallographic analysis. We prepared nano-sized in vivo–grown crystals of Trypanosoma brucei enzymes and applied the emerging method of fre...

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Main Authors: Koopmann, Rudolf, Cupelli, Karolina, Redecke, Lars, Nass, Karol, DePonte, Daniel P, White, Thomas A, Stellato, Francesco, Rehders, Dirk, Liang, Mengning, Andreasson, Jakob, Aquila, Andrew, Bajt, Sasa, Barthelmess, Miriam, Barty, Anton, Bogan, Michael J, Bostedt, Christoph, Boutet, Sébastien, Bozek, John D, Caleman, Carl, Coppola, Nicola, Davidsson, Jan, Doak, R Bruce, Ekeberg, Tomas, Epp, Sascha W, Erk, Benjamin, Fleckenstein, Holger, Foucar, Lutz, Graafsma, Heinz, Gumprecht, Lars, Hajdu, Janos, Hampton, Christina Y, Hartmann, Andreas, Hartmann, Robert, Hauser, Günter, Hirsemann, Helmut, Holl, Peter, Hunter, Mark S, Kassemeyer, Stephan, Kirian, Richard A, Lomb, Lukas, Maia, Filipe R N C, Kimmel, Nils, Martin, Andrew V, Messerschmidt, Marc, Reich, Christian, Rolles, Daniel, Rudek, Benedikt, Rudenko, Artem, Schlichting, Ilme, Schulz, Joachim, Seibert, M Marvin, Shoeman, Robert L, Sierra, Raymond G, Soltau, Heike, Stern, Stephan, Strüder, Lothar, Timneanu, Nicusor, Ullrich, Joachim, Wang, Xiaoyu, Weidenspointner, Georg, Weierstall, Uwe, Williams, Garth J, Wunderer, Cornelia B, Fromme, Petra, Spence, John C H, Stehle, Thilo, Chapman, Henry N, Betzel, Christian, Duszenko, Michael
Format: Artigo
Jezik:Inglês
Izdano: 2012
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Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3429599/
https://ncbi.nlm.nih.gov/pubmed/22286384
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1859
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