In vivo protein crystallization opens new routes in structural biology

Protein crystallization in cells has been observed several times in nature. However, owing to their small size these crystals have not yet been used for X-ray crystallographic analysis. We prepared nano-sized in vivo–grown crystals of Trypanosoma brucei enzymes and applied the emerging method of fre...

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Main Authors: Koopmann, Rudolf, Cupelli, Karolina, Redecke, Lars, Nass, Karol, DePonte, Daniel P, White, Thomas A, Stellato, Francesco, Rehders, Dirk, Liang, Mengning, Andreasson, Jakob, Aquila, Andrew, Bajt, Sasa, Barthelmess, Miriam, Barty, Anton, Bogan, Michael J, Bostedt, Christoph, Boutet, Sébastien, Bozek, John D, Caleman, Carl, Coppola, Nicola, Davidsson, Jan, Doak, R Bruce, Ekeberg, Tomas, Epp, Sascha W, Erk, Benjamin, Fleckenstein, Holger, Foucar, Lutz, Graafsma, Heinz, Gumprecht, Lars, Hajdu, Janos, Hampton, Christina Y, Hartmann, Andreas, Hartmann, Robert, Hauser, Günter, Hirsemann, Helmut, Holl, Peter, Hunter, Mark S, Kassemeyer, Stephan, Kirian, Richard A, Lomb, Lukas, Maia, Filipe R N C, Kimmel, Nils, Martin, Andrew V, Messerschmidt, Marc, Reich, Christian, Rolles, Daniel, Rudek, Benedikt, Rudenko, Artem, Schlichting, Ilme, Schulz, Joachim, Seibert, M Marvin, Shoeman, Robert L, Sierra, Raymond G, Soltau, Heike, Stern, Stephan, Strüder, Lothar, Timneanu, Nicusor, Ullrich, Joachim, Wang, Xiaoyu, Weidenspointner, Georg, Weierstall, Uwe, Williams, Garth J, Wunderer, Cornelia B, Fromme, Petra, Spence, John C H, Stehle, Thilo, Chapman, Henry N, Betzel, Christian, Duszenko, Michael
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3429599/
https://ncbi.nlm.nih.gov/pubmed/22286384
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1859
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