Terminal labeling and addition of homopolymer tracts to duplex DNA fragments by terminal deoxynucleotidyl transferase.
Terminal deoxynucleotidyl transferase, which requires a single-stranded DNA primer under the usual assay conditions, can be made to accept double-stranded DNA as primer for the addition of either rNMP or dNMP, if Mg+2 ion is replaced by Co+2 ion. The priming efficiency in the presence of Co+2 ion wi...
Guardat en:
| Publicat a: | Nucleic Acids Res |
|---|---|
| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
1976
|
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC342951/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/775445/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/3.4.863 |
| Etiquetes: |
Sense etiquetes, Sigues el primer a etiquetar aquest registre!
|