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DNA methylase: purification from ascites cells and the effect of various DNA substrates on its activity.
DNA methylase has been purified 405-fold from Krebs II ascites cells. The purified enzyme is homogeneous on SDS-poly acrylamide gel electrophoresis (molecular weight about 80,000) and the only product of the reaction with DNA is 5-methyl cytosine. Both native and denatured DNA are methylated by the...
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| Autores principales: | , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
1976
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| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC342933/ https://ncbi.nlm.nih.gov/pubmed/131936 |
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