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DNA methylase: purification from ascites cells and the effect of various DNA substrates on its activity.
DNA methylase has been purified 405-fold from Krebs II ascites cells. The purified enzyme is homogeneous on SDS-poly acrylamide gel electrophoresis (molecular weight about 80,000) and the only product of the reaction with DNA is 5-methyl cytosine. Both native and denatured DNA are methylated by the...
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| Publicado no: | Nucleic Acids Res |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1976
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| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC342933/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/131936/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/3.3.677 |
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