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The isolation and partial characterization of a new restriction endonuclease from Providencia stuartii.
We describe the isolation of a new class 2 restriction endonuclease from Providencia stuartii 164. Using the procedure of osmotic shock treatment, we have partially purified this enzyme (Pst 1) and have begun preliminary work to characterize its specificity and requirements. Pst 1 requires Mg++ as t...
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| Autori principali: | , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
1976
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| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC342905/ https://ncbi.nlm.nih.gov/pubmed/768923 |
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