Terminal labeling and addition of homopolymer tracts to duplex DNA fragments by terminal deoxynucleotidyl transferase.

Terminal deoxynucleotidyl transferase, which requires a single-stranded DNA primer under the usual assay conditions, can be made to accept double-stranded DNA as primer for the addition of either rNMP or dNMP, if Mg+2 ion is replaced by Co+2 ion. The priming efficiency in the presence of (C leads to...

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Vydáno v:	Nucleic Acids Res
Hlavní autoři:	Roychoudhury, R, Jay, E, Wu, R
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Oxford University Press 1976
Témata:	Research Article
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC342881/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/765970/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/3.1.101
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