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Determination of the 3' terminal nucleotide of DNA fragments

A new method for determining the 3′-terminal nucleotide of a DNA strand is presented. Use is made of the fact that one (and only one) 2′,3′-dideoxyribonucleotide can be added to the 3′-end of a DNA fragment with calf thymus terminal transferase. Addition of more than one nucleotide analog per strand...

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Detalles Bibliográficos
Main Authors: Olson, Kenneth, Harvey, Clifford
Formato: Artigo
Idioma:Inglês
Publicado: 1975
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC342838/
https://ncbi.nlm.nih.gov/pubmed/165468
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