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Detection by electron microscopy of photo-induced denaturation in lambda DNA.

We have used an electron microscope to study localized denatured regions in ultraviolet-irradiated DNA. DNA from bacteriophage lambda was UV-irradiated and then prepared for electron microscopy after fixing in buffered (pH 9.5) formaldehyde solutions at 25 degrees C. The denatured regions observed c...

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Bibliographic Details
Main Authors: Stafford, R S, Allison, D P, Rahn, R O
Format: Artigo
Language:Inglês
Published: 1975
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC342821/
https://ncbi.nlm.nih.gov/pubmed/1121421
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