Restoration by T4 ligase of DNA sequences sensitive to "flush" cleaving restriction enzyme.
Fouteen "flush"-ended segments originate from the action of the restriction endonuclease Hae III of Haemophilus aegiptius on the DNA of the colicinogenic factor ColE 1 (A. Oka and M. Takanami, Nature, 264, 191, 1976). They are joined by the T4 polynucleotide ligase. The reaction can be monitored by...
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| Опубліковано в:: | Nucleic Acids Res |
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| Автори: | , , , |
| Формат: | Artigo |
| Мова: | Inglês |
| Опубліковано: |
Oxford University Press
1977
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| Онлайн доступ: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC342579/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/198743/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/4.7.2467 |
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