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The properties of native and denatured DNA in buoyant rubidium trichloroacetate at neutral pH

Aqueous RbTCA is generally suitable as a buoyant solvent for both native and denatured DNA at neutral pH and room temperature. Native PM-2 DNA II, for example, is buoyant at 3.29 M salt, 25°C; whereas the denatured strands band together at 4.52 M. Two properties of the solvent make this system uniqu...

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Bibliografiske detaljer
Main Authors: Burke, Rae Lyn, Bauer, William R.
Format: Artigo
Sprog:Inglês
Udgivet: 1977
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC342530/
https://ncbi.nlm.nih.gov/pubmed/19731
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