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RNA polymerase from Bacillus subtilis: isolation of core and holo enzyme by DNA-cellulose chromatography.
A new procedure for the purification of B. subtilis RNA polymerase, based on mild lysis of cells, low speed centrifugation, gel filtration, DEAE-Sephadex chromatography and affinity chromatography on DNA-cellulose, yields three forms of enzyme referred here as enzyme A, B and C. As revealed by SDS g...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1977
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC342466/ https://ncbi.nlm.nih.gov/pubmed/405660 |
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