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Cryptic transcripts from a ubiquitous plasmid origin of replication confound tests for cis-regulatory function

A vast amount of research on the regulation of gene expression has relied on plasmid reporter assays. In this study, we show that plasmids widely used for this purpose constitutively produce substantial amounts of RNA from a TATA-containing cryptic promoter within the origin of replication. Readthro...

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Bibliografski detalji
Glavni autori: Lemp, Nathan A., Hiraoka, Kei, Kasahara, Noriyuki, Logg, Christopher R.
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2012
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3424574/
https://ncbi.nlm.nih.gov/pubmed/22618870
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gks451
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