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Fluoresceinylthiocarbamyl-tRNATyr: a useful derivative of tRNATyr (E.coli) for physicochemical studies.

Fluoresceinylthiocarbamyl-tRNATyr (FTC-tRNATyr) is prepared from tRNATyr and fluoresceinisothiogyanate (FITC) under mildly alkaline conditions. Labelling occurs specificly at the base Q of tRNATyr. The modified tRNA is fully active in the aminoacylation assay; when aminoacylated it is recognized by...

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Bibliografski detalji
Glavni autori: Pingoud, A, Kownatzki, R, Maass, G
Format: Artigo
Jezik:Inglês
Izdano: 1977
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC342435/
https://ncbi.nlm.nih.gov/pubmed/14327
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