Fluoresceinylthiocarbamyl-tRNATyr: a useful derivative of tRNATyr (E.coli) for physicochemical studies.

Fluoresceinylthiocarbamyl-tRNATyr (FTC-tRNATyr) is prepared from tRNATyr and fluoresceinisothiogyanate (FITC) under mildly alkaline conditions. Labelling occurs specificly at the base Q of tRNATyr. The modified tRNA is fully active in the aminoacylation assay; when aminoacylated it is recognized by...

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Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Pingoud, A, Kownatzki, R, Maass, G
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 1977
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC342435/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/14327/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/4.2.327
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