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Gel electrophoretic separation of transcription complexes: an assay for RNA polymerase selectivity and a method for promoter mapping.
We describe a method for analyzing ternary transcription complexes, of RNA polymerase, DNA and nascent RNA32 chains, by agarose gel electrophoresis. When the RNA of such complexes is 32P-labelled, a simple comparison of the DNA fluorogram with an autoradiogram identifies transcriptionally active DNA...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1979
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC342352/ https://ncbi.nlm.nih.gov/pubmed/537912 |
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