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RNA-linked nascent DNA pieces in phage T7-infected Escherichia coli. III. Detection of intact primer RNA

RNA-linked DNA fragments of T7-infected Escherichiacoli were labeled with [(32)P]orthophosphate invivo. The RNA segments of the labeled fragments were isolated by degrading the DNA portion with the 3′→ 5′ exonuclease intrinsic to bacteriophage T4 DNA polymerase and fractionated according to net char...

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Detalhes bibliográficos
Main Authors: Ogawa, Tohru, Okazaki, Tuneko
Formato: Artigo
Idioma:Inglês
Publicado em: 1979
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC342332/
https://ncbi.nlm.nih.gov/pubmed/388359
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