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A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
A procedure for extracting plasmid DNA from bacterial cells is described. The method is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day yet yields plasmid DNA which is pure enough to be digestible by restriction enzymes. The principle of the method is select...
Sparad:
| Huvudupphovsmän: | , |
|---|---|
| Materialtyp: | Artigo |
| Språk: | Inglês |
| Publicerad: |
1979
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| Länkar: | https://ncbi.nlm.nih.gov/pmc/articles/PMC342324/ https://ncbi.nlm.nih.gov/pubmed/388356 |
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