Electrophoretic strand separation of long DNAs with poly (U,G) in agarose gels.
We have found that binding of poly(U,G) to single-stranded DNA decreases its mobility in 0.3% agarose gels. Differential binding to the complementary strands of denatured duplex DNA provides a simple method for strand separation. The method is shown to work with bacteriophage lambda DNA, adenovirus...
Guardat en:
| Publicat a: | Nucleic Acids Res |
|---|---|
| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
1978
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| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC342204/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/99728/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/5.8.2743 |
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