Screening of cloned recombinant DNA in bacteria by in situ colony hybridization.
We have developped in situ methods of colony hybridization in which there is no need to replicate colonies one by one prior to hybridization. The best method consists in promoting partial lysis of the colonies on the plates by means of a resident thermoinducible prophage. It appears that colonies ar...
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| Publié dans: | Nucleic Acids Res |
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| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Oxford University Press
1978
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| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC342171/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/353741/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/5.7.2381 |
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