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Enzymatic synthesis of oligodeoxyribonucleotides of defined sequence. Polynucleotide phosphorylase catalysed synthesis using pyrimidine analog-containing deoxyribonucleoside 5'-diphosphates.

The E. coli polynucleotide phosphorylase-catalysed reaction of the deoxynucleoside 5'-diphosphates of 5-methyldeoxycytidine, N4-hydroxydeoxycytidine, deoxyuridine and 5-mercurideoxyuridine with the primers d(pT-T-A-G) and d(pT-T-T-T-T-T) have been studied under conditions where the primer is ex...

詳細記述

保存先:
書誌詳細
主要な著者: Trip, E M, Smith, M
フォーマット: Artigo
言語:Inglês
出版事項: 1978
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC342102/
https://ncbi.nlm.nih.gov/pubmed/351564
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