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Extracellular nucleases of pseudomonas BAL 31. III. Use of the double-strand deoxyriboexonuclease activity as the basis of a convenient method for the mapping of fragments of DNA produced by cleavage with restriction enzymes.

We have previously characterized an extracellular nuclease from Pseudomonas BAL 31 which, in addition to other activities, displays a double-strand exonuclease activity which progressively shortens both strands of linear duplex DNA molecules from both termini. This degradation is accomplished withou...

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Detalles Bibliográficos
Main Authors: Legerski, R J, Hodnett, J L, Gray, H B
Formato: Artigo
Idioma:Inglês
Publicado: 1978
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC342095/
https://ncbi.nlm.nih.gov/pubmed/307230
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