Photochemical crosslinking of transcription complexes with psoralen. I. Covalent attachment of in vitro SV40 nascent RNA to its double-stranded DNA template.

14C-labeled SV40 DNA has been transcribed with E. coli RNA polymerase using 3H-labeled ribonucleotide triphosphates as precursors. The resulting transcription complexes were then photochemically crosslinked with the psoralen derivative, 4'-aminomethyl-4,5', 8-trimethylpsoralen (AMT), at 37...

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Bibliographische Detailangaben
Hauptverfasser: Shen, C K, Hearst, J E
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1978
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC342089/
https://ncbi.nlm.nih.gov/pubmed/206876
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