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Properies of tRNAPhe from yeast carrying a spin label on the 3'-terminal. Interaction with yeast phenylalanyl-tRNA Synthetase and elongation factor Tu from Escherichia coli.

The 2-thioketo function of tRNAPhe-C-s2C-A in which the penultimate cytidine residue is replaced by 20thiocytidine can serve as a site of specific attachment of spin label. By alkylation of tRNAPhe-C-s2C-A with iodoacetamide or its spin label derivatives tRNAPhe-C-(acm)s2C-A or tRNAPheC-(SL)s2C-A ar...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Principais autores: Sprinzl, M, Siboska, G E, Pedersen, J A
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 1978
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC342029/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/205839/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/5.3.861
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