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Preparation and properties of insolubilized restriction endonucleases.
Type II restriction endonucleases Bam HI and Eco RI were covalently coupled to Sepharose. These insolubilized enzymes generated fragment patterns for several viral DNAs identical to those produced by the respective free enzymes. Conditions for optimal activity were similar for both bound and unbound...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1978
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC342015/ https://ncbi.nlm.nih.gov/pubmed/347400 |
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