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Studies of Dynamic Protein-Protein Interactions in Bacteria Using Renilla Luciferase Complementation Are Undermined by Nonspecific Enzyme Inhibition

The luciferase protein fragment complementation assay is a powerful tool for studying protein-protein interactions. Two inactive fragments of luciferase are genetically fused to interacting proteins, and when these two proteins interact, the luciferase fragments can reversibly associate and reconsti...

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Bibliografiske detaljer
Main Authors: Hatzios, Stavroula K., Ringgaard, Simon, Davis, Brigid M., Waldor, Matthew K.
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2012
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3419657/
https://ncbi.nlm.nih.gov/pubmed/22905225
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0043175
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