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Selective Depletion of rRNA Enables Whole Transcriptome Profiling of Archival Fixed Tissue

We report a method for Selective Depletion of abundant RNA (SDRNA) species from Human total RNA isolated from formalin-fixed, paraffin-embedded (FFPE) tissue, here demonstrating removal of ribosomal and mitochondrial transcripts from clinical FFPE tissue RNA archived up to 20 years. Importantly, SDR...

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Detalhes bibliográficos
Main Authors: Morlan, John D., Qu, Kunbin, Sinicropi, Dominick V.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3416766/
https://ncbi.nlm.nih.gov/pubmed/22900061
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0042882
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