One-Step Sequence- and Ligation-Independent Cloning as a Rapid and Versatile Cloning Method for Functional Genomics Studies

We developed one-step sequence- and ligation-independent cloning (SLIC) as a simple, cost-effective, time-saving, and versatile cloning method. Highly efficient and directional cloning can be achieved by direct bacterial transformation 2.5 min after mixing any linearized vector, an insert(s) prepare...

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Autori principali: Jeong, Jae-Yeon, Yim, Hyung-Soon, Ryu, Ji-Young, Lee, Hyun Sook, Lee, Jung-Hyun, Seen, Dong-Seung, Kang, Sung Gyun
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2012
Soggetti:
Methods
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3416421/
https://ncbi.nlm.nih.gov/pubmed/22610439
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00844-12
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