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Identification and validation of reference genes for expression studies in human keratinocyte cell lines treated with and without interferon-γ – a method for qRT-PCR reference gene determination

Based on the exquisite sensitivity, reproducibility, and wide dynamic range of quantitative reverse-transcription real-time polymerase-chain-reaction (qRT-PCR), it is currently the gold standard for gene expression studies. Target gene expression is calculated relative to a stably expressed referenc...

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Detalhes bibliográficos
Main Authors: Riemer, Angelika B., Keskin, Derin B., Reinherz, Ellis L.
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3413313/
https://ncbi.nlm.nih.gov/pubmed/22775998
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1600-0625.2012.01537.x
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