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Label-free Quantification and Shotgun Analysis of Complex Proteomes by One-dimensional SDS-PAGE/NanoLC-MS: EVALUATION FOR THE LARGE SCALE ANALYSIS OF INFLAMMATORY HUMAN ENDOTHELIAL CELLS
To perform differential studies of complex protein mixtures, strategies for reproducible and accurate quantification are needed. Here, we evaluated a quantitative proteomic workflow based on nanoLC-MS/MS analysis on an LTQ-Orbitrap-VELOS mass spectrometer and label-free quantification using the MFPa...
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| Autors principals: | , , , , , , , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
The American Society for Biochemistry and Molecular Biology
2012
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3412980/ https://ncbi.nlm.nih.gov/pubmed/22518033 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M111.015230 |
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