Label-free Quantification and Shotgun Analysis of Complex Proteomes by One-dimensional SDS-PAGE/NanoLC-MS: EVALUATION FOR THE LARGE SCALE ANALYSIS OF INFLAMMATORY HUMAN ENDOTHELIAL CELLS

To perform differential studies of complex protein mixtures, strategies for reproducible and accurate quantification are needed. Here, we evaluated a quantitative proteomic workflow based on nanoLC-MS/MS analysis on an LTQ-Orbitrap-VELOS mass spectrometer and label-free quantification using the MFPa...

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Bibliografiske detaljer
Main Authors: Gautier, Violette, Mouton-Barbosa, Emmanuelle, Bouyssié, David, Delcourt, Nicolas, Beau, Mathilde, Girard, Jean-Philippe, Cayrol, Corinne, Burlet-Schiltz, Odile, Monsarrat, Bernard, Gonzalez de Peredo, Anne
Format: Artigo
Sprog:Inglês
Udgivet: The American Society for Biochemistry and Molecular Biology 2012
Fag:
Technological Innovation and Resources
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3412980/
https://ncbi.nlm.nih.gov/pubmed/22518033
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M111.015230
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