Expression, purification, crystallization and preliminary X-ray analysis of 4-hydroxy-3-methyl-2-­keto-pentanoate aldolase (asHPAL) from Arthrobacter simplex strain AKU 626

4-Hydroxy-3-methyl-2-keto-pentanoate aldolase (asHPAL), an enzyme used in the synthesis of (2S,3R,4S)-4-hydroxyisoleucine, was crystallized in the absence and the presence of 2-ketobutyrate as one of its substrates by the sitting-drop vapour-diffusion method using PEG 400 as a precipitant. Crystals...

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Autors principals: Guo, Linjun, Okai, Masahiko, Mase, Tomoko, Imai, Fabiana Lica, Miyakawa, Takuya, Nagata, Koji, Yamanaka, Hiroyuki, Fujii, Hidemi, Hibi, Makoto, Ogawa, Jun, Tanokura, Masaru
Format: Artigo
Idioma:Inglês
Publicat: International Union of Crystallography 2012
Matèries:
Crystallization Communications
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3412783/
https://ncbi.nlm.nih.gov/pubmed/22869132
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309112028278
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