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Polycistronic co-expression and non-denaturing purification of histone octamers
Histone octamers are the basic building blocks of chromatin and platforms for diverse genetic mechanisms. We report a simple method for preparing recombinant histone octamers by overexpressing all four histones from a single polycistronic vector followed by standard chromatography under native condi...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3412673/ https://ncbi.nlm.nih.gov/pubmed/22617796 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2012.05.006 |
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