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Replacement and insertion of nucleotides at the anticodon loop of E. coli tRNAMetf by ligation of chemically synthesized ribooligonucleotides.

Insertion of the four major nucleotides at the 5'-side of the anticodon triplet of E. coli tRNAMetf was performed by joining of the half molecules obtained by limited digestion with RNase A and the chemically synthesized tetranucleotide pN-C-A-U using RNA ligase. Insertion of U-U at the 5'-side or A...

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Publicat a:Nucleic Acids Res
Autors principals: Doi, T, Yamane, A, Matsugi, J, Ohtsuka, E, Ikehara, M
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 1985
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC341266/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3892480/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/13.10.3685
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