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Sample Pre-Fractionation for Mass Spectrometry Quantification of Low-Abundance Membrane Proteins

Use of stable isotope-labeled full-length proteins as an internal standard prior to multiple reaction monitoring (MRM) analysis enables pre-fractionation of the target proteins and quantification of those low-abundance proteins, which cannot be reached without biological sample enrichment. In terms...

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Detalhes bibliográficos
Main Authors: Wang, Meiyao, Heo, Gun-Young, Omarova, Saida, Pikuleva, Irina A., Turko, Illarion V.
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3412265/
https://ncbi.nlm.nih.gov/pubmed/22607469
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac300587v
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