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Modification of the melting properties of duplex DNA by attachment of a GC-rich DNA sequence as determined by denaturing gradient gel electrophoresis.

The melting behavior of a DNA fragment carrying the mouse beta maj-globin promoter was investigated as a means of establishing procedures for separating DNA fragments differing by any single base substitution using the denaturing gradient gel electrophoresis procedure of Fischer and Lerman (1,2). We...

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Pubblicato in:Nucleic Acids Res
Autori principali: Myers, R M, Fischer, S G, Maniatis, T, Lerman, L S
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 1985
Accesso online:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC341224/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2987873/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/13.9.3111
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