Overproduction and purification of protein P6 of Bacillus subtilis phage phi 29: role in the initiation of DNA replication.
A phi 29 DNA fragment containing gene 6, required for DNA replication, has been cloned in plasmid pPLc28 under the control of the PL promoter of phage lambda. A polypeptide with an electrophoretic mobility close to that of p6 was labelled with 35S-methionine after heat induction. This protein, repre...
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| Published in: | Nucleic Acids Res |
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| Main Authors: | , , , , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Oxford University Press
1985
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| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC341222/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3158884/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/13.9.3083 |
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