Cloning and characterization of the DAS gene encoding the major methanol assimilatory enzyme from the methylotrophic yeast Hansenula polymorpha.

A gene library from the methanol utilizing yeast Hansenula polymorpha, constructed in a lambda Charon4A vector, was used to clone the gene encoding a key methanol assimilating enzyme, dihydroxyacetone synthase (DHAS) by differential plaque hybridization. The nucleotide sequence of the 2106 bp struct...

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Hauptverfasser: Janowicz, Z A, Eckart, M R, Drewke, C, Roggenkamp, R O, Hollenberg, C P, Maat, J, Ledeboer, A M, Visser, C, Verrips, C T
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1985
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC341220/
https://ncbi.nlm.nih.gov/pubmed/2987872
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