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In vitro transcription with extracts of nuclei of Drosophila embryos.
An in vitro transcription system has been developed from 0.3M NaCl extracts of nuclei of Drosophila embryos. Optimal transcription in the Drosophila embryo extract (DEX) was at 5mM MgCl2, 70mM KCl, 25 degrees C and with promoter concentrations of 0,75-1.0 pmol/assay. In vitro transcription from the...
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| Autors principals: | , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
1985
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| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC341189/ https://ncbi.nlm.nih.gov/pubmed/2987864 |
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