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A highly sensitive FRET-based approach reveals secretion of the actin-binding protein toxofilin during Toxoplasma gondii infection

We have utilized a highly sensitive approach based on fluorescence resonance energy transfer (FRET) and β-lactamase (BLA) (Zlokarnik et al., 1998), which we adapted for the detection of Toxoplasma gondii secreted proteins. This assay revealed that the actin-binding protein toxofilin appears to be se...

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Autori principali: Lodoen, Melissa B., Gerke, Christiane, Boothroyd, John C.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2009
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3411547/
https://ncbi.nlm.nih.gov/pubmed/19732057
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1462-5822.2009.01378.x
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