Hexamine cobalt chloride promotes intermolecular ligation of blunt end DNA fragments by T4 DNA ligase.
Hexamine cobalt chloride (HCC) increases the efficiency of blunt end ligation by T4 DNA ligase about 50 fold. Maximum stimulation occurs when standard buffers for ligation are supplemented with 1 mM HCC. All the ligation events are intermolecular regardless of the initial DNA concentration. In the p...
Kaydedildi:
| Yayımlandı: | Nucleic Acids Res |
|---|---|
| Asıl Yazarlar: | , |
| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
Oxford University Press
1985
|
| Online Erişim: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC341130/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4000951/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/13.6.1997 |
| Etiketler: |
Etiket eklenmemiş, İlk siz ekleyin!
|