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Quantitative analysis of in situ hybridization methods for the detection of actin gene expression.
We have implemented an efficient, quantitative approach for the optimization of in situ hybridization using double-stranded recombinant DNA probes. The model system studied was actin mRNA expression in chicken embryonic muscle cultures. Actin and control (pBR322) probes were nick-translated with p32...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1985
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC341111/ https://ncbi.nlm.nih.gov/pubmed/3889842 |
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