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Efficient secretion and purification of human insulin-like growth factor I with a gene fusion vector in Staphylococci.

A novel approach for production of small polypeptides, using a staphylococcal protein A vector, is described. This system is used to express, secrete and purify human insulin-like growth factor I (IGF-I). A fusion protein consisting of protein A and IGF-I is recovered in high yield by passing the cu...

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Autors principals: Nilsson, B, Holmgren, E, Josephson, S, Gatenbeck, S, Philipson, L, Uhlen, M
Format: Artigo
Idioma:Inglês
Publicat: 1985
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC341062/
https://ncbi.nlm.nih.gov/pubmed/3889837
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