Non-radioactive hybridization probes prepared by the chemical labelling of DNA and RNA with a novel reagent, photobiotin.

A photo-activatable analogue of biotin, N-(4-azido-2-nitrophenyl)-N'-(N-d-biotinyl-3-aminopropyl)-N'-methyl-1,3- propanediamine (photobiotin), has been synthesized and used for the rapid and reliable preparation of large amounts of stable, non-radioactive, biotin-labelled DNA and RNA hybri...

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Bibliografiske detaljer
Udgivet i:Nucleic Acids Res
Main Authors: Forster, A C, McInnes, J L, Skingle, D C, Symons, R H
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1985
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC341032/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2582358/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/13.3.745
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