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Non-radioactive hybridization probes prepared by the chemical labelling of DNA and RNA with a novel reagent, photobiotin.

A photo-activatable analogue of biotin, N-(4-azido-2-nitrophenyl)-N'-(N-d-biotinyl-3-aminopropyl)-N'-methyl-1,3- propanediamine (photobiotin), has been synthesized and used for the rapid and reliable preparation of large amounts of stable, non-radioactive, biotin-labelled DNA and RNA hybri...

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Main Authors: Forster, A C, McInnes, J L, Skingle, D C, Symons, R H
格式: Artigo
語言:Inglês
出版: 1985
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC341032/
https://ncbi.nlm.nih.gov/pubmed/2582358
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