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Methylation of thymine residues during oligonucleotide synthesis.
Thymine residues in an oligodeoxyribonucleotide are subject to methylation at N3 by the internucleotide methyl phosphotriester linkages. This alkylation occurs most rapidly in the presence of a strong base such as DBU, but also takes place, at a much slower rate, during oligonucleotide synthesis.
保存先:
| 出版年: | Nucleic Acids Res |
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| 主要な著者: | , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Oxford University Press
1985
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| オンライン･アクセス: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC341015/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4000926/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/13.2.573 |
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