A procedure for selective full length cDNA cloning of specific RNA species.
A method allowing routine establishment of full length and functionally competent cDNA clones of particular mRNAs from small preparations of polyadenylated RNA is described. Pairs of synthetic primers are used for first and second strand synthesis. They include sequences complementary to the 3' term...
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| Veröffentlicht in: | Nucleic Acids Res |
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| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
1987
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| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC340826/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2884622/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/15.10.3987 |
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