Analysis of hepcidin expression: In situ hybridization and quantitative polymerase chain reaction from paraffin sections

AIM: To establish methods for quantitative polymerase chain reaction (PCR) for hepcidin using RNAs isolated from paraffin-embedded sections and in situ hybridization of hepatocellular carcinoma (HCC). METHODS: Total RNA from paraffin-embedded sections was isolated from 68 paraffin-embedded samples o...

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Autores principales: Sakuraoka, Yuhki, Sawada, Tokihiko, Shiraki, Takayuki, Park, Kyunghwa, Sakurai, Yuhichiro, Tomosugi, Naohisa, Kubota, Keiichi
Formato: Artigo
Lenguaje:Inglês
Publicado: Baishideng Publishing Group Co., Limited 2012
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Brief Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3406426/
https://ncbi.nlm.nih.gov/pubmed/22851866
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v18.i28.3727
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