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Exploiting a Natural Auxotrophy for Genetic Selection
We exploited the natural histidine auxotrophy of Francisella species to develop hisD (encodes histidinol dehydrogenase) as a positive selection marker. A shuttle plasmid (pBR103) carrying Escherichia coli hisD and designed for cloning of PCR fragments replicated in both attenuated and highly virulen...
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| Hoofdauteurs: | , , |
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| Formaat: | Artigo |
| Taal: | Inglês |
| Gepubliceerd in: |
American Society for Microbiology
2012
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| Onderwerpen: | |
| Online toegang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3406110/ https://ncbi.nlm.nih.gov/pubmed/22660718 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00762-12 |
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