Rapid and Specific Detection of Lassa Virus by Reverse Transcription-PCR Coupled with Oligonucleotide Array Hybridization

To facilitate sequence-specific detection of DNA amplified in a diagnostic reverse transcription (RT)-PCR for Lassa virus, we developed an array featuring 47 oligonucleotide probes for post-PCR hybridization of the amplicons. The array procedure may be performed with low-tech equipment and does not...

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Bibliographic Details
Main Authors: Ölschläger, Stephan, Günther, Stephan
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2012
Subjects:
Virology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3405593/
https://ncbi.nlm.nih.gov/pubmed/22535985
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00998-12
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